Short oligoalanine helical peptides for supramolecular nanopore assembly and protein cytosolic delivery.

ABSTRACT: In this work we report a rational design strategy for the identification of new peptide prototypes for the non-disruptive supramolecular permeation of membranes and the transport of different macromolecular giant cargos. The approach targets a maximal enhancement of helicity in the presence of membranes with sequences bearing the minimal number of cationic and hydrophobic moieties. The here reported folding enhancement in membranes allowed the selective non-lytic translocation of different macromolecular cargos including giant proteins. The transport of different high molecular weight polymers and functional proteins was demonstrated in vesicles and in cells with excellent efficiency and optimal viability. As a proof of concept, functional monoclonal antibodies were transported for the fir

SUBMITTER: Pazo M 

PROVIDER: S-EPMC8341679 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature