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One-pot fluorescent assay for sensitive detection of APOBEC3A activity.


ABSTRACT: We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.

SUBMITTER: Wang B 

PROVIDER: S-EPMC8341802 | biostudies-literature |

REPOSITORIES: biostudies-literature

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