Quaternary Structure and Hetero-Oligomerization of Recombinant Human Small Heat Shock Protein HspB7 (cvHsp).
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ABSTRACT:
SUBMITTER: Muranova LK
PROVIDER: S-EPMC8345930 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Publications
Quaternary Structure and Hetero-Oligomerization of Recombinant Human Small Heat Shock Protein HspB7 (cvHsp).
Muranova Lydia K LK Shatov Vladislav M VM Slushchev Andrey V AV Gusev Nikolai B NB
International journal of molecular sciences 20210721 15
In this study, a reliable and simple method of untagged recombinant human HspB7 preparation was developed. Recombinant HspB7 is presented in two oligomeric forms with an apparent molecular weight of 36 kDa (probably dimers) and oligomers with an apparent molecular weight of more than 600 kDa. By using hydrophobic and size-exclusion chromatography, we succeeded in preparation of HspB7 dimers. Mild oxidation promoted the formation of large oligomers, whereas the modification of Cys 126 by iodoacet ...[more]
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