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Using Off-the-Shelf Graphic Design Software for Validating the Operation of an Image Processing System.

ABSTRACT: Fluorescent markers are widely used to protect banknotes, passports, and other documents. Verification of such documents relies upon visual assessment of the markers revealed by ultraviolet (UV) radiation. However, such an explicit approach is inappropriate in certain circumstances, e.g., when discretely checking people for marks left by a pepper gel thrower. The UV light and fluorescent light must not be visible in such applications, yet reliable detection of the markers must still be performed. This problem was successfully resolved using TRIZ methodology, which led to a patent application. The main idea of the solution is to use low-intensity time-variable UV light for illuminating an object and process the image of the object acquired by a camera to detect colour changes too small to be noticed with the naked eye. This paper describes how popular graphics editors such as Adobe Photoshop Elements were used to validate the system concept devised. Simulation experiments used images taken in both visible and UV light to assess the effectiveness and perceptibility of the detection process. The advantage of such validation comes from using commodity software and performing the experiments without access to a laboratory and without physical samples, which makes this approach especially suitable in pandemic times.

SUBMITTER: Chrzaszcz J

PROVIDER: S-EPMC8347455 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Time-lapse microscopy offers a powerful approach for analyzing cellular activity. In particular, this technique is valuable for assessing the behavior of bacterial populations, which can exhibit growth and intercellular interactions in a monolayer. Such time-lapse imaging typically generates large quantities of data, limiting the options for manual investigation. Several image-processing software packages have been developed to facilitate analysis. It can thus be a challenge to identify the software package best suited to a particular research goal. Here, we compare four software packages that support the analysis of 2D time-lapse images of cellular populations: CellProfiler, SuperSegger-Omnipose, DeLTA, and FAST. We compare their performance against benchmarked results on time-lapse observations of Escherichia coli populations. Performance varies across the packages, with each of the four outperforming the others in at least one aspect of the analysis. Not surprisingly, the packages that have been in development for longer showed the strongest performance. We found that deep learning-based approaches to object segmentation outperformed traditional approaches, but the opposite was true for frame-to-frame object tracking. We offer these comparisons, together with insight into usability, computational efficiency, and feature availability, as a guide to researchers seeking image-processing solutions.ImportanceTime-lapse microscopy provides a detailed window into the world of bacterial behavior. However, the vast amount of data produced by these techniques is difficult to analyze manually. We have analyzed four software tools designed to process such data and compared their performance, using populations of commonly studied bacterial species as our test subjects. Our findings offer a roadmap to scientists, helping them choose the right tool for their research. This comparison bridges a gap between microbiology and computational analysis, streamlining research efforts.

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Using Off-the-Shelf Graphic Design Software for Validating the Operation of an Image Processing System.

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