An efficient sorghum transformation system using embryogenic calli derived from mature seeds.

ABSTRACT: Significant progress has been made on sorghum transformation in the last decades; however, the transformation process has been constrained by the availability of immature embryos because most of the researchers have utilized immature embryos as favorable explants. Although immature embryos have been proven to be optimal for tissue culture and transformation, isolation of immature embryos is time-consuming, labor-intensive, and limited by warm weather. In this study, we developed an efficient genetic transformation system using mature seeds as explants. The nptII and gus gene, used as the selective marker and report gene respectively, have been co-transformed by particle bombardment. After optimization of tissue culture, the G418 concentration, and transgenic, the average tran

SUBMITTER: Wang L 

PROVIDER: S-EPMC8349514 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature