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A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions.

ABSTRACT: With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2017, 2020).

SUBMITTER: Madhavan BK

PROVIDER: S-EPMC8350334 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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A laser-mediated photo-manipulative toolbox for generation and real-time monitoring of DNA lesions.

Madhavan Bindhu K BK Han Zhe Z Sickmann Albert A Pepperkok Rainer R Nawroth Peter P PP Kumar Varun V

STAR protocols 20210802 3

With the advancement of laser-based microscopy tools, it is now possible to explore mechano-kinetic processes occurring inside the cell. Here, we describe the advanced protocol for studying the DNA repair kinetics in real time using the laser to induce the DNA damage. This protocol can be used for inducing, testing, and studying the repair mechanisms associated with DNA double-strand breaks, interstrand cross-link repair, and single-strand break repair. For complete details on the use and execut ...[more]

PMID: 34401774

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