Unknown

Dataset Information

0

Unexpected Discovery of Hypermutator Phenotype Sounds the Alarm for Quality Control Strains.


ABSTRACT: Microbial strains with high genomic stability are particularly sought after for testing the quality of commercial microbiological products, such as biological media and antibiotics. Yet, using mutation-accumulation experiments and de novo assembled complete genomes based on Nanopore long-read sequencing, we find that the widely used quality-control strain Shewanella putrefaciens ATCC-8071, also a facultative pathogen, is a hypermutator, with a base-pair substitution mutation rate of 2.42 × 10-8 per nucleotide site per cell division, ∼146-fold greater than that of the wild-type strain CGMCC-1.6515. Using complementation experiments, we confirm that mutL dysfunction, which was a recent evolutionary event, is the cause for the high mutation rate of ATCC-8071. Further analyses also give insight into possible relationships between mutation and genome evolution in this important bacterium. This discovery of a well-known strain being a hypermutator necessitates screening the mutation rate of bacterial strains before any quality control or experiments.

SUBMITTER: Wu K 

PROVIDER: S-EPMC8350357 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3407141 | biostudies-other
| S-EPMC7996481 | biostudies-literature
| S-EPMC7869093 | biostudies-literature
| S-EPMC6986749 | biostudies-literature
| S-EPMC4336291 | biostudies-literature
| S-EPMC9426924 | biostudies-literature
| S-EPMC2849367 | biostudies-literature
| S-EPMC3620903 | biostudies-literature
| S-EPMC3319683 | biostudies-literature
| S-EPMC3386880 | biostudies-other