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Redox index of Cys-thiol residues of serum apolipoprotein E and its diagnostic potential.

ABSTRACT: The redox modulation of Cys-thiol participates in various pathophysiological processes. We explored the proper index for estimating the redox status of Cys-thiol of serum apolipoprotein E (apoE), named "redox-IDX-apoE," which is necessary to understand the redox biology of age-related diseases. The fractions of the reduced form (red-), reversible oxidized form (roxi-), and irreversibly oxidized form (oxi-) apoE in serum, obtained from the patients with no apparent disease (controls, n=192) and with atherosclerosis and type 2 diabetes (patients, n=16), were measured by a band-shift assay using a maleimide compound. Redox-IDX-apoE candidates were determined by calculating the values of these fractions and the total apoE concentration. Cys number of apoE significantly increased for the ratio of roxi-apoE to total-apoE (roxi/total) (E2/E3>E3/E3>E3/E4) but decreased for the ratios of red-apoE to roxi-apoE (red/roxi) and [red-apoE + oxi-apoE] to roxi-apoE ([red + oxi]/roxi) (E2/E3

SUBMITTER: Yamauchi K

PROVIDER: S-EPMC8350432 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature

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Redox index of Cys-thiol residues of serum apolipoprotein E and its diagnostic potential.

Yamauchi Kazuyoshi K Taira Chiaki C Kawakami Yasushi Y

Bioscience reports 20210801 8

<h4>Background</h4>The redox modulation of Cys-thiol participates in various pathophysiological processes. We explored the proper index for estimating the redox status of Cys-thiol of serum apolipoprotein E (apoE), named "redox-IDX-apoE," which is necessary to understand the redox biology of age-related diseases.<h4>Methods</h4>The fractions of the reduced form (red-), reversible oxidized form (roxi-), and irreversibly oxidized form (oxi-) apoE in serum, obtained from the patients with no appare ...[more]

PMID: 34286848

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