Dataset Information

Protective role of 17β-estradiol treatment in renal injury on female rats submitted to brain death.

ABSTRACT:

Background

Clinical and experimental data highlight the consequences of brain death on the quality of organs and demonstrate the importance of donor state to the results of transplantation. Female rats show higher cardio-pulmonary injury linked to decreased concentrations of female sex hormones after brain-dead (BD). This study evaluated the effect of 17β-estradiol on brain death induced renal injury in female rats.

Methods

Female Wistar rats were randomically allocated into 4 groups: false-operation (Sham), BD, treatment with 17β-estradiol (50 µg/mL, 2 mL/h) 3 h after brain death (E2-T3), or immediately after brain death confirmation (E2-T0). Creatinine, urea, cytokines, and complement system components were quantified. Renal injury markers, such as KIM-1, Caspase-3, BCL-2 and MMP2/9 were evaluated.

Results

Brain death leads to increased kidney KIM-1 expression and longer 17β-estradiol treatment resulted in downregulation (P<0.0001). There was increase of neutrophil numbers in kidney from BD rats and E2 treatment was able to reduce it (P=0.018). Regarding complement elements, E2-T3 group evidenced E2 therapeutic effects, reducing C5b-9 (P=0.0004), C3aR (P=0.054) and C5aR (P=0.019). In parallel, there were 17β-estradiol effects in reducing MMP2 (P=0.0043), MMP9 (P=0.011), and IL-6 (P=0.024). Moreover, E2-T3 group improved renal function in comparison to BD group (P=0.0938).

Conclusions

17β-estradiol treatment was able to reduce acute kidney damage in BD female rats owing to its ability to prevent tissue damage, formation of C5b-9, and local synthesis of inflammatory mediators.

SUBMITTER: Armstrong-Jr R

PROVIDER: S-EPMC8350685 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Protective role of 17β-estradiol treatment in renal injury on female rats submitted to brain death.

Armstrong-Jr Roberto R Ricardo-da-Silva Fernanda Yamamoto FY Vidal-Dos-Santos Marina M Correia Cristiano de Jesus CJ Anunciação Lucas Ferreira LF Coutinho E Silva Raphael Dos Santos RDS Moreira Luiz Felipe Pinho LFP Leuvenink Henri Gerrit Derk HGD Breithaupt-Faloppa Ana Cristina AC

Annals of translational medicine 20210701 14

<h4>Background</h4>Clinical and experimental data highlight the consequences of brain death on the quality of organs and demonstrate the importance of donor state to the results of transplantation. Female rats show higher cardio-pulmonary injury linked to decreased concentrations of female sex hormones after brain-dead (BD). This study evaluated the effect of 17β-estradiol on brain death induced renal injury in female rats.<h4>Methods</h4>Female Wistar rats were randomically allocated into 4 gro ...[more]

PMID: 34430566

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Dataset Information

Protective role of 17β-estradiol treatment in renal injury on female rats submitted to brain death.

Background

Methods

Results

Conclusions

Publications

Protective role of 17β-estradiol treatment in renal injury on female rats submitted to brain death.

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