GAS6 ameliorates advanced age-associated meiotic defects in mouse oocytes by modulating mitochondrial function.

ABSTRACT: Previously, we reported that the silencing of growth arrest-specific gene 6 (Gas6) expression in oocytes impairs cytoplasmic maturation by suppressing mitophagy and inducing mitochondrial dysfunction, resulting in fertilization failure. Here, we show that oocyte aging is accompanied by an increase in meiotic defects associated with chromosome misalignment and abnormal spindle organization. Intriguingly, decreased Gas6 mRNA and protein expression were observed in aged oocytes from older females. We further explored the effect of GAS6 on the quality and fertility of aged mouse oocytes using a GAS6 rescue analysis. After treatment with the GAS6 protein, aged oocytes matured normally to the meiosis II (MII) stage. Additionally, maternal age-related meiotic defects were red

SUBMITTER: Kim KH 

PROVIDER: S-EPMC8351714 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature