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Preparation of single cell suspensions enriched in mouse brain vascular cells for single-cell RNA sequencing.

ABSTRACT: Cerebral blood vessels supply oxygen and nutrients, remove metabolic waste, and play a critical role in maintaining brain homeostasis. Cerebrovasculature is composed of heterogeneous populations of brain vascular cells (BVCs). A major challenge in effective cerebrovascular transcriptional profiling is high-quality BVC procurement, permitting high sequencing depth. Here, we establish cell isolation procedures for glio-vascular cell-enriched single-cell RNA sequencing enabling unbiased characterization of BVC transcriptional heterogeneity. Our approach can be used to address vascular-specific contribution to brain diseases. For complete details on the use and execution of this protocol, please refer to Yamazaki et al. (2021).

SUBMITTER: Yamazaki A

PROVIDER: S-EPMC8353357 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Preparation of single cell suspensions enriched in mouse brain vascular cells for single-cell RNA sequencing.

Yamazaki Akari A Shue Francis F Yamazaki Yu Y Martens Yuka A YA Bu Guojun G Liu Chia-Chen CC

STAR protocols 20210805 3

Cerebral blood vessels supply oxygen and nutrients, remove metabolic waste, and play a critical role in maintaining brain homeostasis. Cerebrovasculature is composed of heterogeneous populations of brain vascular cells (BVCs). A major challenge in effective cerebrovascular transcriptional profiling is high-quality BVC procurement, permitting high sequencing depth. Here, we establish cell isolation procedures for glio-vascular cell-enriched single-cell RNA sequencing enabling unbiased characteriz ...[more]

PMID: 34401781

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