ABSTRACT: One new genus (Sestakovaia gen. nov.; Liocranidae) and 10 new species of five families of spiders are described from different provinces of Iran: Brigitteaavicenna sp. nov. (♂♀, Alborz and Kurdistan provinces) (Dictynidae), Micariaatropatene sp. nov. (♂, East Azerbaijan Province), Zagrotesborna sp. nov. (♂, Hormozgan Province), Z.parla sp. nov. (♂, Kerman Province) (Gnaphosidae), Sestakovaiahyrcania sp. nov. (♂, Golestan Province), Mesioteluspatricki sp. nov. (♂, Golestan Province) (Liocranidae), Palpimanuscarmania sp. nov. (♂, Kerman Province), P.persicus sp. nov. (♂♀, Hormozgan Province) (Palpimanidae), Rhysodromusgenoensis sp. nov. (♂, Hormozgan Province), and R.medes sp. nov. (♂, Hormozgan Province) (Philodromidae). Furthermore, Sestakovaiaannulipes (Kulczyński, 1897), comb. nov. (ex. Mesiotelus) and Zagrotesbifurcatus (Zamani, Chatzaki, Esyunin & Marusik, 2021), comb. nov. (ex. Berinda) are proposed as new combinations.