Transition from static culture to stirred tank bioreactor for the allogeneic production of therapeutic discogenic cell spheres.

ABSTRACT:

Background

Culturing cells as cell spheres results in a tissue-like environment that drives unique cell phenotypes, making it useful for generating cell populations intended for therapeutic use. Unfortunately, common methods that utilize static suspension culture have limited scalability, making commercialization of such cell therapies challenging. Our team is developing an allogeneic cell therapy for the treatment of lumbar disc degeneration comprised of discogenic cells, which are progenitor cells expanded from human nucleus pulposus cells that are grown in a sphere configuration.

Methods

We evaluate sphere production in Erlenmeyer, horizontal axis wheel, stirred tank bioreactor, and rocking bag format. We then explore the use of ramped agitation profiles and computational

SUBMITTER: Rodriguez-Granrose D 

PROVIDER: S-EPMC8359559 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature