Human myeloid-derived suppressor cell expansion during sepsis is revealed by unsupervised clustering of flow cytometric data.

ABSTRACT: Myeloid-derived suppressor cells (MDSCs) are important regulators of immune processes during sepsis in mice. However, confirming these observations in humans has been challenging due to the lack of defined preparation protocols and phenotyping schemes for MDSC subsets. Thus, it remains unclear how MDSCs are involved in acute sepsis and whether they have a role in the long-term complications seen in survivors. Here, we combined comprehensive flow cytometry phenotyping with unsupervised clustering using self-organizing maps to identify the three recently defined human MDSC subsets in blood from severe sepsis patients, long-term sepsis survivors, and age-matched controls. We demonstrated the expansion of monocytic M-MDSCs and polymorphonuclear PMN-MDSCs, but not early-stage (e)-MDSCs during a

SUBMITTER: De Zuani M 

PROVIDER: S-EPMC8360154 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature