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Bioformulation of Silk-Based Coating to Preserve and Deliver Rhizobium tropici to Phaseolus vulgaris Under Saline Environments.

ABSTRACT: Seed priming has been for a long time an efficient application method of biofertilizers and biocontrol agents. Due to the quick degradation of the priming agents, this technique has been limited to specific immediate uses. With the increase of awareness of the importance of sustainable use of biofertilizers, seed coating has presented a competitive advantage regarding its ability to adhere easily to the seed, preserve the inoculant, and decompose in the soil. This study compared primed Phaseolus vulgaris seeds with Rhizobium tropici and trehalose with coated seeds using a silk solution mixed with R. tropici and trehalose. We represented the effect of priming and seed coating on seed germination and the development of seedlings by evaluating physiological and morphological parameters under different salinity levels (0, 20, 50, and 75 mM). Results showed that germination and morphological parameters have been significantly enhanced by applying R. tropici and trehalose. Seedlings of coated seeds show higher root density than the freshly primed seeds and the control. The physiological response has been evaluated through the stomatal conductance, the chlorophyll content, and the total phenolic compounds. The stability of these physiological traits indicated the role of trehalose in the protection of the photosystems of the plant under low and medium salinity levels. R. tropici and trehalose helped the plant mitigate the negative impact of salt stress on all traits. These findings represent an essential contribution to our understanding of stress responses in coated and primed seeds. This knowledge is essential to the design of coating materials optimized for stressed environments. However, further progress in this area of research must anticipate the development of coatings adapted to different stresses using micro and macro elements, bacteria, and fungi with a significant focus on biopolymers for sustainable agriculture and soil microbiome preservation.

SUBMITTER: Mhada M

PROVIDER: S-EPMC8366584 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:We studied symbiotic performance of factorial combinations of diverse rhizobial genotypes (GR) and East African common bean varieties (GL) that comprise Andean and Mesoamerican genetic groups. An initial wide screening in modified Leonard jars (LJ) was followed by evaluation of a subset of strains and genotypes in pots (contained the same, sterile medium) in which fixed nitrogen was also quantified. An additive main effect and multiplicative interaction (AMMI) model was used to identify the contribution of individual strains and plant genotypes to the GL × GR interaction. Strong and highly significant GL × GR interaction was found in the LJ experiment but with little evidence of a relation to genetic background or growth habits. The interaction was much weaker in the pot experiment, with all bean genotypes and Rhizobium strains having relatively stable performance. We found that R. etli strain CFN42 and R. tropici strains CIAT899 and NAK91 were effective across bean genotypes but with the latter showing evidence of positive interaction with two specific bean genotypes. This suggests that selection of bean varieties based on their response to inoculation is possible. On the other hand, we show that symbiotic performance is not predicted by any a priori grouping, limiting the scope for more general recommendations. The fact that the strength and pattern of GL × GR depended on growing conditions provides an important cautionary message for future studies.IMPORTANCE The existence of genotype-by-strain (GL × GR) interaction has implications for the expected stability of performance of legume inoculants and could represent both challenges and opportunities for improvement of nitrogen fixation. We find that significant genotype-by-strain interaction exists in common bean (Phaseolus vulgaris L.) but that the strength and direction of this interaction depends on the growing environment used to evaluate biomass. Strong genotype and strain main effects, combined with a lack of predictable patterns in GL × GR, suggests that at best individual bean genotypes and strains can be selected for superior additive performance. The observation that the screening environment may affect experimental outcome of GL × GR means that identified patterns should be corroborated under more realistic conditions.

Project description:BACKGROUND: Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 are ?-Proteobacteria that establish nitrogen-fixing symbioses with a range of legume hosts. These strains are broadly used in commercial inoculants for application to common bean (Phaseolus vulgaris) in South America and Africa. Both strains display intrinsic resistance to several abiotic stressful conditions such as low soil pH and high temperatures, which are common in tropical environments, and to several antimicrobials, including pesticides. The genetic determinants of these interesting characteristics remain largely unknown. RESULTS: Genome sequencing revealed that CIAT 899 and PRF 81 share a highly-conserved symbiotic plasmid (pSym) that is present also in Rhizobium leucaenae CFN 299, a rhizobium displaying a similar host range. This pSym seems to have arisen by a co-integration event between two replicons. Remarkably, three distinct nodA genes were found in the pSym, a characteristic that may contribute to the broad host range of these rhizobia. Genes for biosynthesis and modulation of plant-hormone levels were also identified in the pSym. Analysis of genes involved in stress response showed that CIAT 899 and PRF 81 are well equipped to cope with low pH, high temperatures and also with oxidative and osmotic stresses. Interestingly, the genomes of CIAT 899 and PRF 81 had large numbers of genes encoding drug-efflux systems, which may explain their high resistance to antimicrobials. Genome analysis also revealed a wide array of traits that may allow these strains to be successful rhizosphere colonizers, including surface polysaccharides, uptake transporters and catabolic enzymes for nutrients, diverse iron-acquisition systems, cell wall-degrading enzymes, type I and IV pili, and novel T1SS and T5SS secreted adhesins. CONCLUSIONS: Availability of the complete genome sequences of CIAT 899 and PRF 81 may be exploited in further efforts to understand the interaction of tropical rhizobia with common bean and other legume hosts.

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Bioformulation of Silk-Based Coating to Preserve and Deliver Rhizobium tropici to Phaseolus vulgaris Under Saline Environments.

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