Inflammation in children with cystic fibrosis: contribution of bacterial production of long-chain fatty acids.

ABSTRACT:

Background

Cystic fibrosis (CF) affects >70,000 people worldwide, yet the microbiologic trigger for pulmonary exacerbations (PExs) remains unknown. The objective of this study was to identify changes in bacterial metabolic pathways associated with clinical status.

Methods

Respiratory samples were collected at hospital admission for PEx, end of intravenous (IV) antibiotic treatment, and follow-up from 27 hospitalized children with CF. Bacterial DNA was extracted and shotgun DNA sequencing was performed. MetaPhlAn2 and HUMAnN2 were used to evaluate bacterial taxonomic and pathway relative abundance, while DESeq2 was used to evaluate differential abundance based on clinical status.

Results

The mean age of study participants was 10 years; 85% received combination IV antib

SUBMITTER: Felton E 

PROVIDER: S-EPMC8370878 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature