NoisyR: enhancing biological signal in sequencing datasets by characterizing random technical noise.

ABSTRACT: High-throughput sequencing enables an unprecedented resolution in transcript quantification, at the cost of magnifying the impact of technical noise. The consistent reduction of random background noise to capture functionally meaningful biological signals is still challenging. Intrinsic sequencing variability introducing low-level expression variations can obscure patterns in downstream analyses. We introduce noisyR, a comprehensive noise filter to assess the variation in signal distribution and achieve an optimal information-consistency across replicates and samples; this selection also facilitates meaningful pattern recognition outside the background-noise range. noisyR is applicable to count matrices and sequencing data; it outputs sample-specific signal/noise thresholds and filtered ex

SUBMITTER: Moutsopoulos I 

PROVIDER: S-EPMC8373073 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature