Establishment of the reference intervals of whole blood neutrophil phagocytosis by flow cytometry.

ABSTRACT:

Objective

To investigate the reference intervals (RIs) of the whole blood neutrophil phagocytosis by flow cytometry (FCM) and to study the application value of neutrophil phagocytosis in infectious diseases.

Methods

Pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923) cultured for 18-24 h were labeled by fluorescence probe carboxyfluorescein diacetate succinimidyl ester (CFDA-SE), and then incubated with whole blood at 37℃. The phagocytosis of pathogens by neutrophils was detected by flow cytometry, and a reference interval was established.

Results

In the healthy adults, the reference interval for the neutrophil phagocytosis to Escherichia coli was 46.91%-83.09% and to Staphylococcus aureus was 33.92%-69.48%. This method showed good reproducibilit

SUBMITTER: Yang F 

PROVIDER: S-EPMC8373350 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature