Liquid chromatography mass spectrometry-based proteomics of Escherichia coli single colony.

ABSTRACT: The Escherichia coli proteome is the most extensively characterized and studied of all prokaryotic proteomes. Despite this, large scale bacterial proteomics experiments performed on E. coli cells grown in liquid cultures have failed to identify key virulence factors thought to be important determinants in establishing bacterial infections. It seems likely that many important determinants associated with virulence and host cell adhesion are exclusively expressed during growth in biofilms, which can be crudely mimicked on solid media. This method describes a simple workflow to characterize the unique proteome signature of individual, isolated single colonies, using E. coli K12 strain grown on solid media as a model system. The workflow thus provides a means to explore th

SUBMITTER: Fortuin S 

PROVIDER: S-EPMC8374269 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature