Grimontia sedimenti sp. nov., isolated from benthic sediments near coral reefs south of Kuwait.

ABSTRACT: A Gram-stain-negative, rod and rod-curved shaped motile bacterium designated strain S25^T was obtained from benthic sediment collected near the Kubbar Island coral reefs south of Kuwait. Phenotypic analysis revealed that strain S25^T was slightly halophilic, mesophilic and facultative anaerobic, fermenting d-glucose, d-ribose, d-mannose, d-mannitol, maltose, fructose, gentiobiose, cellobiose, melibiose, trehalose and sucrose. It was positive for oxidase and indole production and negative for arginine dihydrolase and lysine and ornithine decarboxylases. It contained C_16 : 1  ω7c/C_16 : 1  ω6c (summed feature 3), C_18 : 1 ω7c (summed feature 8) and C_16 : 0 as the major fatty acids. Strain S25^T grew optimally at 30 °C and pH 8 in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA sequences revealed that strain S25^T is related to species of the genus Grimontia, having 99.15 % similarity to 'Grimontia indica' AK16^T, 99.08 % to Grimontia celer 96-237^T and 98.66 % to Grimontia marina IMCC 5001^T. The DNA G+C content was 48.8 mol% and the full genome analysis for the strain S25^T showed that the bacterium has a genome size of 5 158 621 bp and contains 4730 predicted protein-encoding genes. The average nucleotide identity values between the S25^T genome and the genomes of its nearest matches ranged between 81.39 and 94.16 %. The strain was distinguishable from the phylogenetically related genera through differences in several phenotypic properties. On the basis of the phenotypic, phylogenetic and genetic data, strain S25^T represents a novel species in the genus Grimontia, for which the name Grimontia sedimenti sp. nov. is proposed. The type strain of Grimontia sedimenti is S25^T (=DSM 28878^T=LMG 28315^T).

SUBMITTER: Mahmoud H 

PROVIDER: S-EPMC8375428 | biostudies-literature |

REPOSITORIES: biostudies-literature