Expression and Purification of the GRAS Domain of Os-SCL7 from Rice for Structural Studies.

ABSTRACT: GRAS proteins, named after the first three members GAI, RGA and SRC, has been found in 294 embryophyta species and is represented by 1,035 sequences. They belong to a plant-specific protein family and play essential roles in plant growth and development. Proteins in this family are defined as minimally containing a conserved GRAS domain, which is about 350-450 resides and can be subdivided into five distinct motifs with their name derived from the most prominent amino acids: LRI (leucine-rich region I), VHIID, LRII (leucine-rich region II), PFYRE and SAW and mainly function in the interaction between GRAS proteins and their partners ( Sun et al., 2012 ).By phylogenetic analysis, the GRAS family can be divided into more than ten subfamilies, of which SCL4/7 is one important subgroup and functions in response to environmental stresses. Here we describe a detailed protocol for the expression and purification of the GRAS domain of Os-SCL7, a SCL4/7 member in rice, which enables us to crystallize it and determine its structure.

SUBMITTER: Li S 

PROVIDER: S-EPMC8376513 | biostudies-literature |

REPOSITORIES: biostudies-literature