Measurement of RNA-induced PKR Activation in vitro.
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ABSTRACT:
SUBMITTER: Yuen KC
PROVIDER: S-EPMC8376569 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Publications
Protein kinase R (PKR) is one of the key RNA-activated sensors for innate immunity. PKR is activated by pathogenic or aberrant RNAs such as short double-stranded RNAs or those with imperfect secondary structures, as well as a reduction in the amount and number of RNA modifications. Activation of PKR may be an underlying mechanism for the pathogenesis of human diseases. In this protocol, I describe a method for studying levels of RNA-induced PKR activation <i>in vitro</i>. ...[more]
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