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Adult rat myelin enhances axonal outgrowth from neural stem cells.

ABSTRACT: Axon regeneration after spinal cord injury (SCI) is attenuated by growth inhibitory molecules associated with myelin. We report that rat myelin stimulated the growth of axons emerging from rat neural progenitor cells (NPCs) transplanted into sites of SCI in adult rat recipients. When plated on a myelin substrate, neurite outgrowth from rat NPCs and from human induced pluripotent stem cell (iPSC)-derived neural stem cells (NSCs) was enhanced threefold. In vivo, rat NPCs and human iPSC-derived NSCs extended greater numbers of axons through adult central nervous system white matter than through gray matter and preferentially associated with rat host myelin. Mechanistic investigations excluded Nogo receptor signaling as a mediator of stem cell-derived axon growth in response to myelin. Transcriptomic screens of rodent NPCs identified the cell adhesion molecule neuronal growth regulator 1 (Negr1) as one mediator of permissive axon-myelin interactions. The stimulatory effect of myelin-associated proteins on rodent NPCs was developmentally regulated and involved direct activation of the extracellular signal-regulated kinase (ERK). The stimulatory effects of myelin on NPC/NSC axon outgrowth should be investigated further and could potentially be exploited for neural repair after SCI.

SUBMITTER: Poplawski GHD

PROVIDER: S-EPMC8377986 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Adult CNS Myelin Enhances Axonal Outgrowth From Neural Stem Cells

Project description:The growth of neural stem cells is strongly enhanced on myelin in vitro and in vivo. To identify the mechanisms associated with this phenome, we compared RNA expression from mouse E12 derived spinal cord cells on stimulation substrates (laminin and myelin) to permissive substrates (PDL). We identified Negr1 as mediator of the stimulatory effects of myelin.

2018-05-16 | GSE98974 | GEO

Myelin basic protein enhances axonal regeneration from neural progenitor cells.

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The proteome of neural stem cells from adult rat hippocampus.

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Cell-Cell Connection Enhances Proliferation and Neuronal Differentiation of Rat Embryonic Neural Stem/Progenitor Cells.

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