Salvaging high-quality genomes of microbial species from a meromictic lake using a hybrid sequencing approach.
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ABSTRACT:
SUBMITTER: Chen YH
PROVIDER: S-EPMC8382752 | biostudies-literature | 2021 Aug
REPOSITORIES: biostudies-literature
Publications
Salvaging high-quality genomes of microbial species from a meromictic lake using a hybrid sequencing approach.
Chen Yu-Hsiang YH Chiang Pei-Wen PW Rogozin Denis Yu DY Degermendzhy Andrey G AG Chiu Hsiu-Hui HH Tang Sen-Lin SL
Communications biology 20210823 1
Most of Earth's bacteria have yet to be cultivated. The metabolic and functional potentials of these uncultivated microorganisms thus remain mysterious, and the metagenome-assembled genome (MAG) approach is the most robust method for uncovering these potentials. However, MAGs discovered by conventional metagenomic assembly and binning are usually highly fragmented genomes with heterogeneous sequence contamination. In this study, we combined Illumina and Nanopore data to develop a new workflow to ...[more]
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