An optimized genetically encoded dual reporter for simultaneous ratio imaging of Ca²⁺ and H⁺ reveals new insights into ion signaling in plants.

ABSTRACT: Whereas the role of calcium ions (Ca²⁺ ) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined. Here we developed CapHensor, an optimized dual-reporter for simultaneous Ca²⁺ and pH ratio-imaging and studied signaling events in pollen tubes (PTs), guard cells (GCs), and mesophyll cells (MCs). Monitoring spatio-temporal relationships between membrane voltage, Ca²⁺ - and pH-dynamics revealed interconnections previously not described. In tobacco PTs, we demonstrated Ca²⁺ -dynamics lag behind pH-dynamics during oscillatory growth, and pH correlates more with growth than Ca²⁺ . In GCs, we demonstrated abscisic acid (ABA) to initiate stomatal closure via rapid cytosolic alkalization followe

SUBMITTER: Li K 

PROVIDER: S-EPMC8383442 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature