Unknown

Dataset Information

0

Evaluation of the Lipid-binding Properties of Recombinant Dystrophin Spectrin-like Repeat Domains R1-3.


ABSTRACT: Recombinant micro-dystrophin genes are designed to treat Duchenne muscular dystrophy (DMD) by retaining dystrophin domains believed to play key functional roles while fitting the packaging capacity of adeno-associated virus vectors. Domains R1-3 are important for muscle force generation and for association with the sarcolemma, but the nature of this interaction is not fully understood. We measured lipid-binding affinity of 3 peptides containing different spectrin-like repeat modules (R1-3; R1-2; and R1, 2, 22). Lipid-binding affinity was highest with R1-3, suggesting that the complete R1-R3 region could be beneficial and should be considered for inclusion in micro-dystrophin constructs.

SUBMITTER: Cooper-Olson G 

PROVIDER: S-EPMC8385511 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC2689041 | biostudies-literature
| S-EPMC5216612 | biostudies-literature
| S-EPMC10612232 | biostudies-literature
| S-EPMC7725318 | biostudies-literature
| S-EPMC3365720 | biostudies-literature
| S-EPMC3416534 | biostudies-literature
| S-EPMC2689040 | biostudies-literature
| S-EPMC2659240 | biostudies-literature
| S-EPMC1751415 | biostudies-literature
| S-EPMC1367010 | biostudies-literature