Glutathione reductase gsr-1 is an essential gene required for Caenorhabditis elegans early embryonic development.

ABSTRACT: Glutathione is the most abundant thiol in the vast majority of organisms and is maintained in its reduced form by the flavoenzyme glutathione reductase. In this work, we describe the genetic and functional analysis of the Caenorhabditis elegans gsr-1 gene that encodes the only glutathione reductase protein in this model organism. By using green fluorescent protein reporters we demonstrate that gsr-1 produces two GSR-1 isoforms, one located in the cytoplasm and one in the mitochondria. gsr-1 loss of function mutants display a fully penetrant embryonic lethal phenotype characterized by a progressive and robust cell division delay accompanied by an aberrant distribution of interphasic chromatin in the periphery of the cell nucleus. Maternally expressed GSR-1 is sufficient to support embryonic

SUBMITTER: Mora-Lorca JA 

PROVIDER: S-EPMC8386055 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature