Ontology highlight
ABSTRACT:
SUBMITTER: Lin L
PROVIDER: S-EPMC8387396 | biostudies-literature | 2021 Aug
REPOSITORIES: biostudies-literature
Lin Lin L Chen Junyu J Mitra Ruchira R Gao Quanxiu Q Cheng Feiyue F Xu Tong T Zuo Zhenqiang Z Xiang Hua H Han Jing J
Communications biology 20210825 1
The haloarchaeon Haloferax mediterranei is a potential strain for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) production, yet the production yield and cost are the major obstacles hindering the use of this archaeal strain. Leveraging the endogenous type I-B CRISPR-Cas system in H. mediterranei, we develop a CRISPR-based interference (CRISPRi) approach that allows to regulate the metabolic pathways related to PHBV synthesis, thereby enhancing PHBV production. Our CRISPRi approach can down ...[more]