Dataset Information

Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus).

ABSTRACT: In this study, internal organs (tongue, intestine, and spleen) of 23 free-ranging Italian wolves (Canis lupus italicus) found dead between 2017 and 2019 were tested for Carnivore protoparvovirus 1, Canine adenovirus (CAdV), and Canine circovirus (CanineCV) using real-time PCR assays. Genetic characterisation of the identified viruses was carried out by amplification, sequencing, and analysis of the complete viral genome or informative viral genes. All the wolves tested positive for at least one of the DNA viruses screened, and 11/23 were coinfected. Carnivore protoparvoviruses were the most frequently detected viruses (21/23), followed by CanineCV (11/23) and CAdV (4/23). From the analysis of the partial VP2 gene of 13 carnivore protoparvoviruses, 12 were canine parvovirus type 2b, closely related to the strains detected in dogs and wild carnivores from Italy, and one was a feline panleukopenia-like virus. Of the four CAdV identified, two were CAdV-1 and two were CAdV-2. The complete genome of seven CanineCVs was sequenced and related to the CanineCV identified in dogs, wolves, and foxes worldwide. Close correlations emerged between the viruses identified in wolves and those circulating in domestic dogs. Further studies are needed to investigate if these pathogens may be potentially cross-transmitted between the two species.

SUBMITTER: Balboni A

PROVIDER: S-EPMC8388400 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus).

Balboni Andrea A Urbani Lorenza L Delogu Mauro M Musto Carmela C Fontana Maria Cristina MC Merialdi Giuseppe G Lucifora Giuseppe G Terrusi Alessia A Dondi Francesco F Battilani Mara M

Animals : an open access journal from MDPI 20210724 8

In this study, internal organs (tongue, intestine, and spleen) of 23 free-ranging Italian wolves (Canis lupus italicus) found dead between 2017 and 2019 were tested for Carnivore protoparvovirus 1, Canine adenovirus (CAdV), and Canine circovirus (CanineCV) using real-time PCR assays. Genetic characterisation of the identified viruses was carried out by amplification, sequencing, and analysis of the complete viral genome or informative viral genes. All the wolves tested positive for ...[more]

PMID: 34438655

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Project description:BackgroundThe only natural hosts of Pseudorabies virus (PRV) are members of the family Suidae (Sus scrofa scrofa). In mammals, the infection is usually fatal and typically causes serious neurologic disease. This study describes four Aujeszky's disease cases in free-ranging Italian wolves (Canis lupus italicus). In Italy, the wolf is a strictly protected species and is in demographic expansion.Case presentationThree wolves (Wolf A, B, and C) were found in a regional park in Northern Italy, and one (Wolf D) was found in Central Italy. Wolf A and D were alive at the time of the finding and exhibited a fatal infection with epileptic seizures and dyspnoea, dying after a few hours. Wolf B presented scratching lesions under the chin and a detachment of the right earlobe, whilst Wolf C was partially eaten. The wolves showed hepatic congestion, diffuse enteritis, moderate pericardial effusion, severe bilateral pneumonia, and diffuse hyperaemia in the brain. The diagnostic examinations included virological analyses and detection of toxic molecules able to cause serious neurological signs. All four wolves tested positive for pseudorabies virus (PrV). The analysed sequences were placed in Italian clade 1, which is divided into two subclades, "a" and "b". The sequences of Wolf A, B, and C were closely related to other Italian sequences in the subclade b, originally obtained from wild boars and hunting dogs. The sequence from Wolf D was located within the same clade and was closely related to the French hunting dog sequences belonging to group 4.ConclusionResults showed the presence of PrV strains currently circulating in wild boars and free-ranging Italian wolves. The genetic characterisation of the PrV UL44 sequences from the four wolves confirmed the close relationship with the sequences from wild boars and hunting dogs. This fact supports a possible epidemiological link with the high PrV presence in wild boars and the possibility of infection in wolves through consumption of infected wild boar carcasses or indirect transmission. To the best of our knowledge, this study is the first detection of Pseudorabies virus in free-ranging Italian wolves in northern and central Italy.

Project description:Simple Summary Prosocial behaviour is shown by a variety of mammals. Prosocial behaviours can also be part of social feedback mechanisms. For group-living mammals, group coordination and group cohesion are crucial factors for survival. Therefore, group-living mammals display a broad variety of social behaviours towards their group members. Canidae are known to be socially organised mammals. Wolves are one of the most cooperative and social canine species. In this case study, we investigated social feedback mechanisms in two European grey wolf groups. The social feedback was observed after novel object interaction, bold behaviour, and the individual behaviours of single individuals. The purpose of this paper is to focus on prosocial behaviour that might serve as social reward. We assume that there is social reward behaviour as a category that falls within social feedback in grey wolves. Abstract Prosociality occurs in many species and is likely to be a crucial factor for the survival of group-living animals. Social feedback is an important mechanism for the coordination of group decisions. Since group-living animals with specific personality axes, i.e., boldness, are known to provide certain benefits for their group, bold actions might receive more prosocial feedback than other actions. Our case study aims to determine whether bold behaviour, i.e., novel object interaction (Nobj), might be answered more frequently with prosocial behaviours. We investigated the differences in the frequency of occurrence in prosocial behaviours after three different individual actions in two groups of grey wolves. We aim to outline the development of a social reward behavioural category as part of social feedback mechanisms. We used Markov chain models for probability analyses, and a non-parametric ANOVA to test for differences between the influences of individual behaviours on the probability of a prosocial behaviour chain. We additionally tested for the potential influences of age, sex and personality on the frequency of Nobj. Our results suggest that bold interactions are more often responded to with prosocial behaviour. Bold behaviour might be more often socially rewarded because of its benefits for group-living animals. More research is needed to investigate whether bold behaviour is more frequently responded to prosocially, and to investigate the social reward phenomenon.

Dataset Information

Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (Canis lupus italicus).

Publications

Integrated Use of Molecular Techniques to Detect and Genetically Characterise DNA Viruses in Italian Wolves (<i>Canis lupus italicus</i>).

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