Unknown

Dataset Information

0

Diversity of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle from Central and Southern Chile.


ABSTRACT: Cattle are the main reservoir of Shiga toxin-producing Escherichia coli (STEC), one of the world's most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces from central and southern Chile. We analyzed 446 cattle fecal samples and isolated non-O157 STEC from 12.6% (56/446); a total of 93 different isolates were recovered. Most isolates displayed β-glucuronidase activity (96.8%; 90/93) and fermented sorbitol (86.0%; 80/93), whereas only 39.8% (37/93) were resistant to tellurite. A subgroup of 30 representative non-O157 STEC isolates was selected for whole-genome sequencing and bioinformatics analysis. In silico analysis showed that they grouped into 16 different sequence types and 17 serotypes; the serotypes most frequently identified were O116:H21 and O168:H8 (13% each). A single isolate of serotype O26:H11 was recovered. One isolate was resistant to tetracycline and carried resistance genes tet(A) and tet(R); no other isolate displayed antimicrobial resistance or carried antimicrobial resistance genes. The intimin gene (eae) was identified in 13.3% (4/30) of the genomes and 90% (27/30) carried the stx2 gene. A phylogenetic reconstruction demonstrated that the isolates clustered based on serotypes, independent of geographical origin. These results indicate that cattle in Chile carry a wide diversity of STEC potentially pathogenic for humans based on the presence of critical virulence genes.

SUBMITTER: Diaz L 

PROVIDER: S-EPMC8388633 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3408066 | biostudies-literature
| S-EPMC8553066 | biostudies-literature
| S-EPMC8249597 | biostudies-literature
| S-EPMC7828786 | biostudies-literature
| S-EPMC4806951 | biostudies-literature
| S-EPMC8106712 | biostudies-literature
| S-EPMC4366395 | biostudies-literature
| S-EPMC5852577 | biostudies-literature
| S-EPMC6728992 | biostudies-literature
| S-EPMC4542925 | biostudies-literature