D-Galacturonic acid reduction by S. cerevisiae for L-galactonate production from extracted sugar beet press pulp hydrolysate.

ABSTRACT: Pectin-rich residues are considered as promising feedstocks for sustainable production of platform chemicals. Enzymatic hydrolysis of extracted sugar beet press pulp (SBPP) releases the main constituent of pectin, D-galacturonic acid (D-GalA). Using engineered Saccharomyces cerevisiae, D-GalA is then reduced to L-galactonate (L-GalOA) with sorbitol as co-substrate. The current work addresses the combination of enzymatic hydrolysis of pectin in SBPP with a consecutive optimized biotransformation of the released D-GalA to L-GalOA in simple batch processes in stirred-tank bioreactors. Process conditions were first identified with synthetic media, where a product concentration of 9.9 g L^-1 L-GalOA was obtained with a product selectivity of 99% (L-GalOA D-GalA^-1) at pH 5 w

SUBMITTER: Wagner J 

PROVIDER: S-EPMC8390429 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature