VEGF Detection via Simplified FLISA Using a 3D Microfluidic Disk Platform.

ABSTRACT: Fluorescence-linked immunosorbent assay (FLISA) is a commonly used, quantitative technique for detecting biochemical changes based on antigen-antibody binding reactions using a well-plate platform. As the manufacturing technology of microfluidic system evolves, FLISA can be implemented onto microfluidic disk platforms which allows the detection of trace biochemical reactions with high resolutions. Herein, we propose a novel microfluidic system comprising a disk with a three-dimensional incubation chamber, which can reduce the amount of the reagents to 1/10 and the required time for the entire process to less than an hour. The incubation process achieves an antigen-antibody binding reaction as well as the binding of fluorogenic substrates to target proteins. The FLISA protocol in the 3D inc

SUBMITTER: Kang DH 

PROVIDER: S-EPMC8393963 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature