Dataset Information

Upregulation of 15 Antisense Long Non-Coding RNAs in Osteosarcoma.

ABSTRACT: The human genome encodes thousands of natural antisense long noncoding RNAs (lncRNAs); they play the essential role in regulation of gene expression at multiple levels, including replication, transcription and translation. Dysregulation of antisense lncRNAs plays indispensable roles in numerous biological progress, such as tumour progression, metastasis and resistance to therapeutic agents. To date, there have been several studies analysing antisense lncRNAs expression profiles in cancer, but not enough to highlight the complexity of the disease. In this study, we investigated the expression patterns of antisense lncRNAs from osteosarcoma and healthy bone samples (24 tumour-16 bone samples) using RNA sequencing. We identified 15 antisense lncRNAs (RUSC1-AS1, TBX2-AS1, PTOV1-AS1, UBE2D3-AS1, ERCC8-AS1, ZMIZ1-AS1, RNF144A-AS1, RDH10-AS1, TRG-AS1, GSN-AS1, HMGA2-AS1, ZNF528-AS1, OTUD6B-AS1, COX10-AS1 and SLC16A1-AS1) that were upregulated in tumour samples compared to bone sample controls. Further, we performed real-time polymerase chain reaction (RT-qPCR) to validate the expressions of the antisense lncRNAs in 8 different osteosarcoma cell lines (SaOS-2, G-292, HOS, U2-OS, 143B, SJSA-1, MG-63, and MNNG/HOS) compared to hFOB (human osteoblast cell line). These differentially expressed IncRNAs can be considered biomarkers and potential therapeutic targets for osteosarcoma.

SUBMITTER: Rothzerg E

PROVIDER: S-EPMC8394133 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Upregulation of 15 Antisense Long Non-Coding RNAs in Osteosarcoma.

Rothzerg Emel E Ho Xuan Dung XD Xu Jiake J Wood David D Märtson Aare A Kõks Sulev S

Genes 20210726 8

The human genome encodes thousands of natural antisense long noncoding RNAs (lncRNAs); they play the essential role in regulation of gene expression at multiple levels, including replication, transcription and translation. Dysregulation of antisense lncRNAs plays indispensable roles in numerous biological progress, such as tumour progression, metastasis and resistance to therapeutic agents. To date, there have been several studies analysing antisense lncRNAs expression profiles in cancer, but no ...[more]

PMID: 34440306

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Project description:BackgroundNumerous studies have reported the relationship between Long non-coding RNAs (LncRNAs) expression and prognosis of osteosarcoma, but less consensus has been reached. Our meta-analysis was conducted to quantitatively assess the relationship between the expression of LncRNAs, prognosis and clinical pathology in osteosarcoma development.MethodsPubMed,Embase,Web of Science,The Cochrane Library,SionMed,CNKI and WanFang databases were carefully searched to identify eligible studies. The pooled hazard ratios (HRs) and 95% confidence intervals (CIs) were calculated to evaluate the prognostic significance of LncRNAs expression in osteosarcoma. Moreover, meta-regression analysis and subgroup analysis were carried out to explore the potential sources of heterogeneity.ResultsA total of 20 studies comprising 1749 patients were included in present meta-analysis. The results showed that the over-expression of LncRNA had a significant correlation with overall survival (OS) (HR = 2.16, 95% CI:1.68-2.79), and was not related to disease free survival (DFS) (HR = 0.71, 95% CI:0.05-9.53). Subgroup analysis further indicated that LncRNA transcription level was significantly associated with alkaline phosphatase (HR = 2.13, 95% CI:1.58-2.88) , tumor size (< 8/ ≥ 8:HR = 1.97, 95% CI: 1.55-2.62) , metastasis (yes/no: HR = 2.14,95% CI:1.15-3.97) , distant metastasis(presence/absence: HR = 4.02, 95% CI:3.05-5.23) and Enneking stage(IIA /IIB-III:HR = 3.2, 95% CI:2.48-4.14), but not associated with age (≤ 25/ > 25:HR = 1.01, 95% CI:0.78-1.3), gender(female/male: HR = 1.15, 95% CI: 0.96-1.37), tumor site (femur,tibia/elsewhere:HR = 1.15, 95% CI:0.94-1.4) and chemotherapy (yes/no: HR = 1.45, 95% CI:0.46-4.63).ConclusionsThis study demonstrated that abnormal LncRNAs expression might be potential prognostic markers to predict worse overall survival in osteosarcoma patients. However, the cut-off values may be the source of heterogeneity.

Dataset Information

Upregulation of 15 Antisense Long Non-Coding RNAs in Osteosarcoma.

Publications

Upregulation of 15 Antisense Long Non-Coding RNAs in Osteosarcoma.

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