Identification of TSPAN4 as Novel Histamine H₄ Receptor Interactor.

ABSTRACT: The histamine H₄ receptor (H₄R) is a G protein-coupled receptor that is predominantly expressed on immune cells and considered to be an important drug target for various inflammatory disorders. Like most GPCRs, the H₄R activates G proteins and recruits β-arrestins upon phosphorylation by GPCR kinases to induce cellular signaling in response to agonist stimulation. However, in the last decade, novel GPCR-interacting proteins have been identified that may regulate GPCR functioning. In this study, a split-ubiquitin membrane yeast two-hybrid assay was used to identify H₄R interactors in a Jurkat T cell line cDNA library. Forty-three novel H₄R interactors were identified, of which 17 have also been previously observed in MYTH screens to int

SUBMITTER: Ma X 

PROVIDER: S-EPMC8394291 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature