Implementation of an Extraction-Free COVID Real-Time PCR Workflow in a Pediatric Hospital Setting.

ABSTRACT:

Background

This study outlines the development, implementation, and impact of a laboratory-developed, extraction-free real-time PCR assay as the primary diagnostic test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a pediatric hospital.

Methods

Clinical specimens from both upper and lower respiratory tract sources were validated, including nasopharyngeal aspirates, nasopharyngeal swabs, anterior nares swabs, and tracheal aspirates (n = 333 clinical samples). Testing volumes and laboratory turnaround times were then compared before and after implementation to investigate effects of the workflow changes.

Results

Compared to magnetic-bead extraction platforms, extraction-free real-time PCR demonstrated ≥95% positive agreement and ≥97% negative agree

SUBMITTER: Dumm RE 

PROVIDER: S-EPMC8394822 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature