Unknown

Dataset Information

0

Ageing Causes Ultrastructural Modification to Calcium Release Units and Mitochondria in Cardiomyocytes.


ABSTRACT: Ageing is associated with an increase in the incidence of heart failure, even if the existence of a real age-related cardiomyopathy remains controversial. Effective contraction and relaxation of cardiomyocytes depend on efficient production of ATP (handled by mitochondria) and on proper Ca2+ supply to myofibrils during excitation-contraction (EC) coupling (handled by Ca2+ release units, CRUs). Here, we analyzed mitochondria and CRUs in hearts of adult (4 months old) and aged (≥24 months old) mice. Analysis by confocal and electron microscopy (CM and EM, respectively) revealed an age-related loss of proper organization and disposition of both mitochondria and EC coupling units: (a) mitochondria are improperly disposed and often damaged (percentage of severely damaged mitochondria: adults 3.5 ± 1.1%; aged 16.5 ± 3.5%); (b) CRUs that are often misoriented (longitudinal) and/or misplaced from the correct position at the Z line. Immunolabeling with antibodies that mark either the SR or T-tubules indicates that in aged cardiomyocytes the sarcotubular system displays an extensive disarray. This disarray could be in part caused by the decreased expression of Cav-3 and JP-2 detected by western blot (WB), two proteins involved in formation of T-tubules and in docking SR to T-tubules in dyads. By WB analysis, we also detected increased levels of 3-NT in whole hearts homogenates of aged mice, a product of nitration of protein tyrosine residues, recognized as marker of oxidative stress. Finally, a detailed EM analysis of CRUs (formed by association of SR with T-tubules) points to ultrastructural modifications, i.e., a decrease in their frequency (adult: 5.1 ± 0.5; aged: 3.9 ± 0.4 n./50 μm2) and size (adult: 362 ± 40 nm; aged: 254 ± 60 nm). The changes in morphology and disposition of mitochondria and CRUs highlighted by our results may underlie an inefficient supply of Ca2+ ions and ATP to the contractile elements, and possibly contribute to cardiac dysfunction in ageing.

SUBMITTER: Di Fonso A 

PROVIDER: S-EPMC8395047 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC6377823 | biostudies-literature
| S-EPMC3538766 | biostudies-literature
| S-EPMC4742110 | biostudies-literature
| S-EPMC6217927 | biostudies-literature
| S-EPMC2862690 | biostudies-literature
| S-EPMC3797627 | biostudies-literature
| S-EPMC1183792 | biostudies-other
| S-EPMC6312737 | biostudies-literature
| S-EPMC4983655 | biostudies-literature
| S-EPMC2678594 | biostudies-literature