Nano-Particles Carried by Multiple Dynein Motors Self-Regulate Their Number of Actively Participating Motors.

ABSTRACT: Intra-cellular active transport by native cargos is ubiquitous. We investigate the motion of spherical nano-particles (NPs) grafted with flexible polymers that end with a nuclear localization signal peptide. This peptide allows the recruitment of several mammalian dynein motors from cytoplasmic extracts. To determine how motor-motor interactions influenced motility on the single microtubule level, we conducted bead-motility assays incorporating surface adsorbed microtubules and combined them with model simulations that were based on the properties of a single dynein. The experimental and simulation results revealed long time trajectories: when the number of NP-ligated motors Nm increased, run-times and run-lengths were enhanced and mean velocities were somewhat decreased. Moreover, the dep

SUBMITTER: Halbi G 

PROVIDER: S-EPMC8396316 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature