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1H, 13C and 15N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2.

ABSTRACT: The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7-33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the 1H, 13C and 15N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair.

SUBMITTER: Richter C 

PROVIDER: S-EPMC8401371 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

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<sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2.

Richter Christian C   Hohmann Katharina F KF   Toews Sabrina S   Mathieu Daniel D   Altincekic Nadide N   Bains Jasleen Kaur JK   Binas Oliver O   Ceylan Betül B   Duchardt-Ferner Elke E   Ferner Jan J   Fürtig Boris B   Grün J Tassilo JT   Hengesbach Martin M   Hymon Daniel D   Jonker Hendrik R A HRA   Knezic Bozana B   Korn Sophie M SM   Landgraf Tom T   Löhr Frank F   Peter Stephen A SA   Pyper Dennis J DJ   Qureshi Nusrat S NS   Schlundt Andreas A   Schnieders Robbin R   Stirnal Elke E   Sudakov Alexey A   Vögele Jennifer J   Weigand Julia E JE   Wirmer-Bartoschek Julia J   Witt Kerstin K   Wöhnert Jens J   Schwalbe Harald H   Wacker Anna A  

Biomolecular NMR assignments 20210828 2

The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7-33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of  ...[more]

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