Unknown

Dataset Information

0

1H, 13C and 15N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2.

ABSTRACT: The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7-33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of the viral life cycle. We present here the 1H, 13C and 15N chemical shift assignment of the 29 nucleotides-RNA construct 5_SL1, which denotes the native 27mer SL1 stabilized by an additional terminal G-C base-pair.

SUBMITTER: Richter C 

PROVIDER: S-EPMC8401371 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

<sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N assignment of stem-loop SL1 from the 5'-UTR of SARS-CoV-2.

Richter Christian C   Hohmann Katharina F KF   Toews Sabrina S   Mathieu Daniel D   Altincekic Nadide N   Bains Jasleen Kaur JK   Binas Oliver O   Ceylan Betül B   Duchardt-Ferner Elke E   Ferner Jan J   Fürtig Boris B   Grün J Tassilo JT   Hengesbach Martin M   Hymon Daniel D   Jonker Hendrik R A HRA   Knezic Bozana B   Korn Sophie M SM   Landgraf Tom T   Löhr Frank F   Peter Stephen A SA   Pyper Dennis J DJ   Qureshi Nusrat S NS   Schlundt Andreas A   Schnieders Robbin R   Stirnal Elke E   Sudakov Alexey A   Vögele Jennifer J   Weigand Julia E JE   Wirmer-Bartoschek Julia J   Witt Kerstin K   Wöhnert Jens J   Schwalbe Harald H   Wacker Anna A  

Biomolecular NMR assignments 20210828 2

The stem-loop (SL1) is the 5'-terminal structural element within the single-stranded SARS-CoV-2 RNA genome. It is formed by nucleotides 7-33 and consists of two short helical segments interrupted by an asymmetric internal loop. This architecture is conserved among Betacoronaviruses. SL1 is present in genomic SARS-CoV-2 RNA as well as in all subgenomic mRNA species produced by the virus during replication, thus representing a ubiquitous cis-regulatory RNA with potential functions at all stages of  ...[more]

Similar Datasets

Unknown <sup>1</sup>H, <sup>13</sup>C, <sup>15</sup>N and <sup>31</sup>P chemical shift assignment for stem-loop 4 from the 5'-UTR of SARS-CoV-2.
| S-EPMC8083917 | biostudies-literature
Unknown <sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N chemical shift assignment of the stem-loops 5b + c from the 5'-UTR of SARS-CoV-2.
| S-EPMC8853908 | biostudies-literature
Unknown &lt;sup&gt;1&lt;/sup&gt;H, &lt;sup&gt;13&lt;/sup&gt;C and &lt;sup&gt;15&lt;/sup&gt;N chemical shift assignment for stem-loop 5a from the 5'UTR of HCoV-229E.
| S-EPMC12513883 | biostudies-literature
Unknown <sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N backbone chemical shift assignments of SARS-CoV-2 nsp3a.
| S-EPMC7819138 | biostudies-literature
Unknown <sup>1</sup>H, <sup>13</sup>C and <sup>15</sup>N backbone resonance assignment of BRCA1 fragment 219-504.
| S-EPMC7462910 | biostudies-literature
Unknown <sup>1</sup>H, <sup>15</sup>N, <sup>13</sup>C resonance assignment of the human CD44 cytoplasmic tail (669-742).
| S-EPMC6439174 | biostudies-literature
Unknown Targeting stem-loop 1 of the SARS-CoV-2 5' UTR to suppress viral translation and Nsp1 evasion.
| S-EPMC8892331 | biostudies-literature
Unknown <sup>1</sup>H, <sup>13</sup>C, <sup>15</sup>N backbone resonance assignment for the 1-164 construct of human XRCC4.
| S-EPMC8481219 | biostudies-literature
Unknown &lt;sup&gt;1&lt;/sup&gt;H, &lt;sup&gt;13&lt;/sup&gt;C, &lt;sup&gt;15&lt;/sup&gt;N and &lt;sup&gt;31&lt;/sup&gt;P chemical shift assignment of the first stem-loop Guanidine-II riboswitch from Escherichia coli.
| S-EPMC12117010 | biostudies-literature
Unknown <sup>1</sup>H, <sup>13</sup>C, and <sup>15</sup>N NMR chemical shift assignment of LytM N-terminal domain (residues 26-184).
| S-EPMC10630248 | biostudies-literature