RNA-dependent RNA Polymerase Assay for Hepatitis E Virus.
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ABSTRACT:
SUBMITTER: Nair VP
PROVIDER: S-EPMC8410430 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Publications
RNA-dependent RNA Polymerase Assay for Hepatitis E Virus.
Nair Vidya P VP Anang Saumya S Srivastava Akriti A Surjit Milan M
Bio-protocol 20170405 7
RNA-dependent RNA polymerase (RdRp) is essential for the replication of viral RNA for RNA viruses. It synthesizes the complementary strand of viral genomic RNA, which is used subsequently as a template to generate more copies of viral genome. This assay measures activity of the hepatitis E virus (HEV) RdRp. In contrast to protocols available to assay the RdRp activity of many other viruses, this assay utilizes DIG-11-UTP as a nonradioactive alternative to <sup>32</sup>P-UTP, thereby increasing t ...[more]
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