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Heat-Stress Responses Differ among Species from Different 'Bangia' Clades of Bangiales (Rhodophyta).

ABSTRACT: The red alga 'Bangia' sp. ESS1, a 'Bangia' 2 clade member, responds to heat stress via accelerated asexual reproduction and acquires thermotolerance based on heat-stress memory. However, whether these strategies are specific to 'Bangia' 2, especially 'Bangia' sp. ESS1, or whether they are employed by all 'Bangia' species is currently unknown. Here, we examined the heat-stress responses of 'Bangia' sp. ESS2, a newly identified 'Bangia' clade 3 member, and Bangia atropurpurea. Intrinsic thermotolerance differed among species: Whereas 'Bangia' sp. ESS1 survived at 30 °C for 7 days, 'Bangia' sp. ESS2 and B. atropurpurea did not, with B. atropurpurea showing the highest heat sensitivity. Under sublethal heat stress, the release of asexual spores was highly repressed in 'Bangia' sp. ESS2 and completely repressed in B. atropurpurea, whereas it was enhanced in 'Bangia' sp. ESS1. 'Bangia' sp. ESS2 failed to acquire heat-stress tolerance under sublethal heat-stress conditions, whereas the acquisition of heat tolerance by priming with sublethal high temperatures was observed in both B. atropurpurea and 'Bangia' sp. ESS1. Finally, unlike 'Bangia' sp. ESS1, neither 'Bangia' sp. ESS2 nor B. atropurpurea acquired heat-stress memory. These findings provide insights into the diverse heat-stress response strategies among species from different clades of 'Bangia'.

SUBMITTER: Khoa HV

PROVIDER: S-EPMC8412102 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BackgroundPyropia haitanensis is an economically important mariculture crop in China and is also valuable in life science research. However, the lack of genetic information of this organism hinders the understanding of the molecular mechanisms of specific traits. Thus, high-throughput sequencing is needed to generate a number of transcriptome sequences to be used for gene discovery and molecular marker development.ResultsIn this study, high-throughput sequencing was used to analyze the global transcriptome of P. haitanensis. Approximately 103 million 90 bp paired-end reads were generated using an Illumina HiSeq 2000. De novo assembly with paired-end information yielded 24,575 unigenes with an average length of 645 bp. Based on sequence similarity searches with known proteins, a total of 16,377 (66.64%) genes were identified. Of these annotated unigenes, 5,471 and 9,168 unigenes were assigned to gene ontology and clusters of orthologous groups, respectively. Searching against the KEGG database indicated that 12,167 (49.51%) unigenes mapped to 124 KEGG pathways. Among the carbon fixation pathways, almost all the essential genes related to the C3- and C4-pathways for P. haitanensis were discovered. Significantly different expression levels of three key genes (Rubisco, PEPC and PEPCK) in different lifecycle stages of P. haitanensis indicated that the carbon fixation pathway in the conchocelis and thallus were different, and the C4-like pathway might play important roles in the conchocelis stage. In addition, 2,727 cSSRs loci were identified in the unigenes. Among them, trinucleotide SSRs were the dominant repeat motif (87.17%, 2,377) and GCC/CCG motifs were the most common repeats (60.07%, 1,638). High quality primers to 824 loci were designed and 100 primer pairs were randomly evaluated in six strains of P. haitanensis. Eighty-seven primer pairs successfully yielded amplicons.ConclusionThis study generated a large number of putative P. haitanensis transcript sequences, which can be used for novel gene discovery and gene expression profiling analyses under different physiological conditions. A number of the cSSR markers identified can be used for molecular markers and will facilitate marker assisted selection in P. haitanensis breeding. These sequences and markers will provide valuable resources for further P. haitanensis studies.

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Heat-Stress Responses Differ among Species from Different 'Bangia' Clades of Bangiales (Rhodophyta).

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