Ontology highlight
ABSTRACT:
SUBMITTER: Tao W
PROVIDER: S-EPMC8413891 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Tao Wanyu W Liu Qing Q Huang Shisheng S Wang Xin X Qu Shiyuan S Guo Junfan J Ou Danfeng D Li Guanglei G Zhang Yu Y Xu Xiangmin X Huang Xingxu X
Molecular therapy. Nucleic acids 20210729
Multi-nucleotide variants (MNVs) represent an important type of genetic variation and have biological and clinical significance. To simulate MNVs, we designed four dual-mutation base editors combining hA3A(Y130F), TadA8e(V106W), and protospacer adjacent motif (PAM)-flexible SpRY and selected cytosine and adenine base editor-SpRY (CABE-RY), which had the best editing performance, for further study. Characterization and comparison showed that CABE-RY had a smaller DNA editing window and lower RNA ...[more]