Dataset Information

Molecular and Pathological Investigations of Selected Viral Neuropathogens in Rabies-Negative Brains of Cats and Dogs Revealed Neurotropism of Carnivore Protoparvovirus-1.

ABSTRACT: Throughout the year, the Thai Red Cross Society (TRCS), Bangkok, Thailand, received more than 100 animals that died of suspected rabies due to neurological clinical signs. Concerning the role of viral infection in the brain in the outcome of neurological diseases in cats and dogs, a comprehensive study was conducted of 107 brain samples of cats and dogs submitted to the TRCS from August 2019 to August 2020. Selective molecular screening using conventional polymerase chain reaction (PCR) and reverse transcription PCR targeting nine viral pathogens was employed in addition to histopathological investigations. The results showed that carnivore protoparvovirus-1 (CPPV-1) was detected in 18.69% of the cats and dogs sampled (20/107). These results were found in young and old animals; the brain tissue did not show any pathological changes suggesting encephalitis or cerebellar hypoplasia. In addition, feline calicivirus, feline alphaherpesvirus-1, feline coronavirus, and canine distemper virus were also detected, providing a broader range of potential viral infections to consider in the clinical manifestation of neurological disorders in companion animals. The detection of all pathogens was confirmed by the localization of each viral antigen in various resident brain cells using immunohistochemistry. A unique L582S amino acid substitution of the non-structural protein 1 gene coding sequence, speculated to be associated with the neurotropism of CPPV-1 in cats and dogs, was not evident. In conclusion, this study revealed a noteworthy neurotropism of CPPV-1 in both cats and dogs without neurological lesions.

SUBMITTER: Wardhani SW

PROVIDER: S-EPMC8416986 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:In recent years, prices for veterinary care have received considerable attention in mainstream media, yet scientific literature has not delved into actual figures. This study aims to elucidate veterinary care costs for dogs, cats, and horses across five countries [Sweden (SE), Norway (NO), Denmark (DK), United Kingdom (UK), and Ireland (IR, with limited data)] through web searches. Utilising online business directories, we located URLs featuring veterinary care prices in autumn 2022, and repeated tri-monthly five times. Vetpris.se (VP), a price comparison site for SE, NO, and DK, emerged from the search. Additionally, we sought to compare price data from veterinary clinics (ranging from animal hospitals to small private clinics) using a similar approach to VP. We targeted elective procedures (e.g., gonadectomy, GDY) and common procedures (e.g., pyometra surgery in dogs). Comparing data from the same clinics' websites and from VP within extraction from autumn 2022 to winter 2023/2024, median prices for dog and cat GDY were largely consistent. By October 2023, median prices for male cat GDY ranged from €72 (SE) to €230 (DK), and €130 (SE) to €361 (DK) for females; for dog GDY from €390 (SE) to €599 (DK) for males, and €461 (UK) to €1015 (DK) for females. Across sources, median prices for cat and dog GDY increased by 2-24% over a year for procedures with at least 10 clinics per extraction. Equine GDY (per sedation and local analgaesia) in SE saw a 64% increase by year-end, with a median price of €492. Emergency surgeries during regular-hours (e.g., pyometra and caesarean section) in SE were approximately €2,300 at the last extraction, marking a 27% increase for pyometra surgery during regular-hours and 15% after-hours compared to the previous year. Variability existed within and across countries and diagnoses/procedures. Cross-validation suggested VP generally provided reliable information, though data points for emergencies were limited. Our web searching tool necessitated extensive manual verification, indicating room for further development. We recommend enhancing price transparency for animal owners to become better informed about the cost of veterinary care and be able to make informed choices.

Dataset Information

Molecular and Pathological Investigations of Selected Viral Neuropathogens in Rabies-Negative Brains of Cats and Dogs Revealed Neurotropism of Carnivore Protoparvovirus-1.

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