Ontology highlight
ABSTRACT:
SUBMITTER: Ghanta KS
PROVIDER: S-EPMC8417391 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Ghanta Krishna S KS Ishidate Takao T Mello Craig C CC
STAR protocols 20210901 3
In <i>Caenorhabditis elegans,</i> targeted genome editing techniques are now routinely used to generate germline edits. The remarkable ease of <i>C. elegans</i> germline editing is attributed to the syncytial nature of the pachytene ovary which is easily accessed by microinjection. This protocol describes the step-by-step details and troubleshooting tips for the entire CRISPR-Cas genome editing procedure, including gRNA design and microinjection of ribonucleoprotein complexes, followed by screen ...[more]