Ontology highlight
ABSTRACT:
SUBMITTER: Caceres-Martell Y
PROVIDER: S-EPMC8420214 | biostudies-literature | 2021 Nov
REPOSITORIES: biostudies-literature
Cáceres-Martell Yaiza Y Fernández-Soto Daniel D Campos-Silva Carmen C García-Cuesta Eva M EM Casasnovas Jose M JM Navas-Herrera David D Beneítez-Martínez Alexandra A Martínez-Fleta Pedro P Alfranca Arantzazu A Sánchez-Madrid Francisco F Escudero-López Gabriela G Vilches Carlos C Jara-Acevedo Ricardo R Reyburn Hugh T HT Rodríguez-Frade José M JM Valés-Gómez Mar M
European journal of immunology 20210819 11
Here, we describe a new, simple, highly multiplexed serological test that generates a more complete picture of seroconversion than single antigen-based assays. Flow cytometry is used to detect multiple Ig isotypes binding to four SARS-CoV-2 antigens: the Spike glycoprotein, its RBD fragment (the main target for neutralizing antibodies), the nucleocapsid protein, and the main cysteine-like protease in a single reaction. Until now, most diagnostic serological tests measured antibodies to only one ...[more]