Unknown

Dataset Information

0

Differential roles of RIG-I like receptors in SARS-CoV-2 infection.


ABSTRACT: Retinoic acid-inducible gene I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5) sense viral RNA and activate antiviral immune responses. Herein we investigate their functions in human epithelial cells, the primary and initial target of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A deficiency in MDA5, RIG-I or mitochondrial antiviral signaling protein (MAVS) enhanced viral replication. The expression of the type I/III interferon (IFN) during infection was impaired in MDA5-/- and MAVS-/-, but not in RIG-I-/-, when compared to wild type (WT) cells. The mRNA level of full-length angiotensin-converting enzyme 2 (ACE2), the cellular entry receptor for SARS-CoV-2, was ~ 2.5-fold higher in RIG-I-/- than WT cells. These data demonstrate MDA5 as the predominant SARS-CoV-2 sensor, IFN-independent induction of ACE2 and anti-SARS-CoV-2 role of RIG-I in epithelial cells.

SUBMITTER: Yang DM 

PROVIDER: S-EPMC8421188 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3331923 | biostudies-literature
| S-EPMC9188443 | biostudies-literature
| S-EPMC8641883 | biostudies-literature
| S-SCDT-10_1038-S44318-024-00061-0 | biostudies-other
| 12454 | ecrin-mdr-crc
2013-07-31 | E-GEOD-47960 | biostudies-arrayexpress
2013-07-31 | E-GEOD-47961 | biostudies-arrayexpress
2013-07-31 | E-GEOD-47962 | biostudies-arrayexpress
| S-SCDT-EMBOJ-2020-106501 | biostudies-other
| S-EPMC8209947 | biostudies-literature