Dataset Information

HOME vs. LAB hair samples for the determination of long-term steroid concentrations: a comparison between hair samples collected by laypersons and trained research staff.

ABSTRACT: Hair segment analysis is a valuable tool for the assessment of cumulative long-term steroid secretion. Preliminary findings suggest comparable cortisol concentrations in hair collected by instructed laypersons and research staff. However, it remains unclear whether hair sample quality and hair steroids other than cortisol are affected by level of experience (laypersons vs. research staff), home collection circumstances (instructions, familiarity to participant, performance confidence), and characteristics of the layperson (conscientiousness). Sixty participants (23.6 ± 3.9 years; 43 females) provided hair samples twice: first collected by laypersons (HOME) according to provided instructions (written vs. written/video-based instructions) and second by trained research staff (LAB) on the same day or the day after the HOME collection. Hair steroid concentrations (cortisol, cortisone, DHEA, progesterone) were determined using LC-MS/MS. Hair sample quality was evaluated using nine predefined criteria. Laypersons completed questionnaires for the assessment of potential factors of hair outcome measures (hair steroid concentrations, hair sample quality). Hair steroids from HOME and LAB samples were positively correlated (rs between 0.76 and 0.89) and did not significantly differ, with the exception of cortisone. The quality of hair samples was significantly higher for LAB than for HOME samples. Neither HOME collection circumstances nor layperson-related characteristics had an impact on hair outcome measures. However, a low self-reported performance confidence predicted a high absolute difference between HOME and LAB DHEA. In summary, our findings suggest higher quality of hair samples collected by trained research staff compared to instructed laypersons. However, these differences might be negligible, considering the high correlation between HOME and LAB hair steroid concentrations, with the characteristics of the layperson or collection circumstances having a minor impact on hair steroids and hair sample quality. These findings provide further support for the notion that well-instructed laypersons can be enabled to collect hair samples.

SUBMITTER: Skoluda N

PROVIDER: S-EPMC8423693 | biostudies-literature |

REPOSITORIES: biostudies-literature

ACCESS DATA

Similar Datasets

Project description:Distal hair segments collected at delivery may allow for the assessment of maternal cortisol secretion in early pregnancy, an important time window for fetal development. Therefore, an investigation of the validity of distal hair cortisol concentrations is warranted. We examined the concordance between proximal and distal hair cortisol concentrations (HCC), both representing the first trimester of pregnancy. The study population was comprised of a random sample of 97 women participating in the Pregnancy Outcomes Maternal and Infant Study, a prospective cohort study of pregnant women attending prenatal clinics in Lima, Peru. Each participant provided two hair samples: once at enrollment [mean gestational age (GA) = 13.1 weeks] and again at full-term delivery (mean GA = 39.0 weeks). Hair segments reflecting the first trimester were: 3 cm hair segments closest to the scalp on the first hair sample (proximal) and 6-9 cm from the scalp on the second hair sample (distal). HCC was determined using Luminescence Immunoassay. A subset (N = 28) had both hair segments additionally analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS). HCC values were log-transformed (logHCC), and proximal-distal differences tested using paired sample t-tests. Concordance was evaluated within and across assay types. LogHCC, measured using immunoassay, in distal hair segments was lower compared to proximal hair segments (1.35 versus 1.64 respectively; p = .02). No difference was observed using LC-MS/MS (1.99 versus 1.83, respectively; p=.33). Proximal-distal concordance was low within assay (immunoassay: Pearson = 0.27 and κ = 0.10; LC-MS/MS: Pearson = 0.37 and κ = 0.07). High correlation was observed across assays for both distal (Pearson = 0.78, p < .001; κ = 0.64) and proximal segments (Pearson = 0.96, p < .001; κ = 0.75). In conclusion, distal first-trimester hair segments collected at delivery have lower absolute HCC compared to HCC in proximal first trimester hair segments collected in early pregnancy, and are poorly concordant with HCC in proximal segments. Findings may inform the design of future studies.

Project description:Although combining genetic and endocrine data from non-invasively collected hair samples has potential to improve the conservation of threatened mammals, few studies have evaluated this opportunity. In this study, we determined if steroid hormone (testosterone, progesterone, estradiol and cortisol) concentration profiles in 169 hair samples collected from free-ranging brown bears (Ursus arctos) could be used to accurately discriminate between immature and adult bears within each sex. Because hair samples were acquired opportunistically, we also needed to establish if interactions between hormones and several non-hormone factors (ordinal day, year, contact method, study area) were associated with age class. For each sex, we first compared a suite of candidate models by Akaike Information Criteria model selection, using different adult-age thresholds (3, 4 and 5 years), to determine the most supported adult age. Because hair hormone levels better reflect the endocrine state at an earlier time, possibly during the previous year, then at the time of sampling, we re-analysed the data, excluding the records for bears at the adult-age threshold, to establish if classification accuracy improved. For both sexes, candidate models were most supported based on a 3-year-old adult-age threshold. Classification accuracy did not improve with the 3-year-old bear data excluded. Male age class was predicted with a high degree of accuracy (88.4%) based on the concomitant concentrations of all four hormones. Female age class was predicted with less accuracy (77.1%) based only on testosterone and cortisol. Accuracy was reduced for females, primarily because we had poor success in correctly classifying immature bears (60%) whereas classification success for adult females was similar to that for males (84.5%). Given the small and unbalanced sample used in this study, our findings should be viewed as preliminary, but they should also provide a basis for more comprehensive future studies.

Dataset Information

HOME vs. LAB hair samples for the determination of long-term steroid concentrations: a comparison between hair samples collected by laypersons and trained research staff.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets