Video-rate large-scale imaging with Multi-Z confocal microscopy.
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ABSTRACT: Fast, volumetric imaging over large scales has been a long-standing challenge in biological microscopy. To address this challenge, we report an augmented variant of confocal microscopy that uses a series of reflecting pinholes axially distributed in the detection space, such that each pinhole probes a different depth within the sample. We thus obtain simultaneous multiplane imaging without the need for axial scanning. Our microscope technique is versatile and configured here to provide two-color fluorescence imaging with a field of view larger than a millimeter at video rate. Its general applicability is demonstrated with neuronal imaging of both Caenorhabditis elegans and mouse brains in vivo.
SUBMITTER: Badon A
PROVIDER: S-EPMC8425499 | biostudies-literature |
REPOSITORIES: biostudies-literature
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