Unknown

Dataset Information

0

Video-rate large-scale imaging with Multi-Z confocal microscopy.


ABSTRACT: Fast, volumetric imaging over large scales has been a long-standing challenge in biological microscopy. To address this challenge, we report an augmented variant of confocal microscopy that uses a series of reflecting pinholes axially distributed in the detection space, such that each pinhole probes a different depth within the sample. We thus obtain simultaneous multiplane imaging without the need for axial scanning. Our microscope technique is versatile and configured here to provide two-color fluorescence imaging with a field of view larger than a millimeter at video rate. Its general applicability is demonstrated with neuronal imaging of both Caenorhabditis elegans and mouse brains in vivo.

SUBMITTER: Badon A 

PROVIDER: S-EPMC8425499 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3475383 | biostudies-literature
| S-EPMC10370169 | biostudies-literature
| S-EPMC3646600 | biostudies-other
| S-EPMC4881331 | biostudies-literature
| S-EPMC6754501 | biostudies-literature
| S-EPMC3670618 | biostudies-literature
| S-EPMC4147922 | biostudies-literature
| S-EPMC8837204 | biostudies-literature
| S-EPMC5945773 | biostudies-literature
| S-EPMC7101442 | biostudies-literature