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Video-rate large-scale imaging with Multi-Z confocal microscopy.

ABSTRACT: Fast, volumetric imaging over large scales has been a long-standing challenge in biological microscopy. To address this challenge, we report an augmented variant of confocal microscopy that uses a series of reflecting pinholes axially distributed in the detection space, such that each pinhole probes a different depth within the sample. We thus obtain simultaneous multiplane imaging without the need for axial scanning. Our microscope technique is versatile and configured here to provide two-color fluorescence imaging with a field of view larger than a millimeter at video rate. Its general applicability is demonstrated with neuronal imaging of both Caenorhabditis elegans and mouse brains in vivo.

SUBMITTER: Badon A

PROVIDER: S-EPMC8425499 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Video-rate large-scale imaging with Multi-Z confocal microscopy.

Badon Amaury A Bensussen Seth S Gritton Howard J HJ Awal Mehraj R MR Gabel Christopher V CV Han Xue X Mertz Jerome J

Optica 20190321 4

Fast, volumetric imaging over large scales has been a long-standing challenge in biological microscopy. To address this challenge, we report an augmented variant of confocal microscopy that uses a series of reflecting pinholes axially distributed in the detection space, such that each pinhole probes a different depth within the sample. We thus obtain simultaneous multiplane imaging without the need for axial scanning. Our microscope technique is versatile and configured here to provide two-color ...[more]

PMID: 34504902

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