Ontology highlight
ABSTRACT:
SUBMITTER: Kuster RD
PROVIDER: S-EPMC8425578 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Kuster Ryan D RD Yencho G Craig GC Olukolu Bode A BA
Briefings in bioinformatics 20210901 5
Next-generation sequencing (NGS) enables massively parallel acquisition of large-scale omics data; however, objective data quality filtering parameters are lacking. Although a useful metric, evidence reveals that platform-generated Phred values overestimate per-base quality scores. We have developed novel and empirically based algorithms that streamline NGS data quality filtering. The pipeline leverages known sequence motifs to enable empirical estimation of error rates, detection of erroneous b ...[more]