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Development and application of a SARS-CoV-2 colorimetric biosensor based on the peroxidase-mimic activity of γ-Fe₂O₃ nanoparticles.

ABSTRACT: A practical colorimetric assay was developed for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). For this purpose, magnetic γ Fe₂O₃ nanoparticles were synthesized and used as a peroxidase-like mimic activity molecule. In the presence of γ Fe₂O₃ nanoparticles, the color change of H₂O₂ included 3,3',5,5'-tetramethylbenzidine was monitored at the wavelength of 654 nm when spike protein interacted with angiotensin-converting enzyme 2 receptor. This oxidation-reduction reaction was examined both spectroscopically and by using electrochemical techniques. The experimental parameters were optimized and the analytical characteristics investigated. The developed assay was applied to real SARS-CoV-2 samples, and very good results that were in accordance with the real time polymerase chain reaction were obtained.

SUBMITTER: Buyuksunetci YT

PROVIDER: S-EPMC8428493 | biostudies-literature |

REPOSITORIES: biostudies-literature

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