Single-Cell Cloning of Breast Cancer Cells Secreting Specific Subsets of Extracellular Vesicles.

ABSTRACT: Extracellular vesicles (EVs) mediate communication in health and disease. Conventional assays are limited in profiling EVs secreted from large populations of cells and cannot map EV secretion onto individual cells and their functional profiles. We developed a high-throughput single-cell technique that enabled the mapping of dynamics of EV secretion. By utilizing breast cancer cell lines, we established that EV secretion is heterogeneous at the single-cell level and that non-metastatic cancer cells can secrete specific subsets of EVs. Single-cell RNA sequencing confirmed that pathways related to EV secretion were enriched in the non-metastatic cells compared with metastatic cells. We established isogenic clonal cell lines from non-metastatic cells with differing propensities for CD81+CD63+E

SUBMITTER: Fathi M 

PROVIDER: S-EPMC8430892 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature